| Autor: |
Minh, Nghiem Ngoc, Van Bac, Nguyen, Son, Nguyen Thai, Lien, Vu Thi Kim, Ha, Chu Hoang, Cuong, Nguyen Huu, Mai, Cung Thi Ngoc, Le, Thanh Hoa |
| Zdroj: |
Journal of Clinical Microbiology; March 2012, Vol. 50 Issue: 3 p598-601, 4p |
| Abstrakt: |
ABSTRACTMolecular characterization of the drug resistance of Mycobacterium tuberculosisstrains with different origins can generate information that is useful for developing molecular methods. These methods are widely applicable for rapid detection of drug resistance. A total of 166 rifampin (RIF)- and/or isoniazid (INH)-resistant strains of M. tuberculosishave been isolated from different parts of Vietnam; they were screened for mutations associated with resistance to these drugs by sequence analysis investigating genetic mutations associated with RIF and INH resistance. Seventeen different mutations were identified in 74 RIF-resistant strains, 56 of which (approximately 76%) had mutations in the so-called 81-bp “hot-spot” region of the rpoBgene. The most common point mutations were in codons 531 (37.8%), 526 (23%), and 516 (9.46%) of the rpoBgene. Mutations were not found in three strains (4.05%). In the case of INH resistance, five different mutations in the katGgenes of 82 resistant strains were detected, among which the nucleotide substitution at codon 315 (76.83%) is the most common mutation. This study provided the first molecular characterization of INH and RIF resistance of M. tuberculosisstrains from Vietnam, and detection of the katGand rpoBmutations of the INH and RIF-resistant strains should be useful for rapid detection of the INH- and RIF-resistant strains by molecular tests. |
| Databáze: |
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