| Autor: |
Fillo, Silvia, Giordani, Francesco, Anniballi, Fabrizio, Gorgé, Olivier, Ramisse, Vincent, Vergnaud, Gilles, Riehm, Julia M., Scholz, Holger C., Splettstoesser, Wolf D., Kieboom, Jasper, Olsen, Jaran-Strand, Fenicia, Lucia, Lista, Florigio |
| Zdroj: |
Journal of Clinical Microbiology; December 2011, Vol. 49 Issue: 12 p4252-4263, 12p |
| Abstrakt: |
ABSTRACTClostridium botulinumis a taxonomic designation that encompasses a broad variety of spore-forming, Gram-positive bacteria producing the botulinum neurotoxin (BoNT). C. botulinumis the etiologic agent of botulism, a rare but severe neuroparalytic disease. Fine-resolution genetic characterization of C. botulinumisolates of any BoNT type is relevant for both epidemiological studies and forensic microbiology. A 10-locus multiple-locus variable-number tandem-repeat analysis (MLVA) was previously applied to isolates of C. botulinumtype A. The present study includes five additional loci designed to better address proteolytic B and F serotypes. We investigated 79 C. botulinumgroup I strains isolated from human and food samples in several European countries, including types A (28), B (36), AB (4), and F (11) strains, and 5 nontoxic Clostridium sporogenes. Additional data were deduced from in silicoanalysis of 10 available fully sequenced genomes. This 15-locus MLVA (MLVA-15) scheme identified 86 distinct genotypes that clustered consistently with the results of amplified fragment length polymorphism (AFLP) and MLVA genotyping in previous reports. An MLVA-7 scheme, a subset of the MLVA-15, performed on a lab-on-a-chip device using a nonfluorescent subset of primers, is also proposed as a first-line assay. The phylogenetic grouping obtained with the MLVA-7 does not differ significantly from that generated by the MLVA-15. To our knowledge, this report is the first to analyze genetic variability among all of the C. botulinumgroup I serotypes by MLVA. Our data provide new insights into the genetic variability of group I C. botulinumisolates worldwide and demonstrate that this group is genetically highly diverse. |
| Databáze: |
Supplemental Index |
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