| Autor: |
Perry, John D., Asir, Kerry, Halimi, Diane, Orenga, Sylvain, Dale, Joanne, Payne, Michelle, Carlton, Ruth, Evans, Jim, Gould, F. Kate |
| Zdroj: |
Journal of Clinical Microbiology; November 2010, Vol. 48 Issue: 11 p3852-3858, 7p |
| Abstrakt: |
ABSTRACTRapid and effective methods for the isolation of Clostridium difficilefrom stool samples are desirable to obtain isolates for typing or to facilitate accurate diagnosis of C. difficile-associated diarrhea. We report on the evaluation of a prototype chromogenic medium (ID C. difficileprototype [IDCd]) for isolation of C. difficile. The chromogenic medium was compared using (i) 368 untreated stool samples that were also inoculated onto CLO medium, (ii) 339 stool samples that were subjected to alcohol shock and also inoculated onto five distinct selective agars, and (iii) standardized suspensions of 10 C. difficileribotypes (untreated and alcohol treated) that were also inoculated onto five distinct selective agars. Two hundred thirty-six isolates of C. difficilewere recovered from 368 untreated stool samples, and all but 1 of these strains (99.6%) were recovered on IDCd within 24 h, whereas 74.6% of isolates were recovered on CLO medium after 48 h. Of 339 alcohol-treated stool samples cultured onto IDCd and five other selective agars, C. difficilewas recovered from 218 samples using a combination of all media. The use of IDCd allowed recovery of 96.3% of isolates within 24 h, whereas 51 to 83% of isolates were recovered within 24 h using the five other media. Finally, when they were challenged with pure cultures, all 10 ribotypes of C. difficilegenerated higher colony counts on IDCd irrespective of alcohol pretreatment or duration of incubation. We conclude that IDCd is an effective medium for isolation of C. difficilefrom stool samples within 24 h. |
| Databáze: |
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