| Autor: |
Ciardo, Diana E., Lucke, Katja, Imhof, Alex, Bloemberg, Guido V., Bo¨ttger, Erik C. |
| Zdroj: |
Journal of Clinical Microbiology; August 2010, Vol. 48 Issue: 8 p2809-2813, 5p |
| Abstrakt: |
ABSTRACTThe implementation of internal transcribed spacer (ITS) sequencing for routine identification of molds in the diagnostic mycology laboratory was analyzed in a 5-year study. All mold isolates (n= 6,900) recovered in our laboratory from 2005 to 2009 were included in this study. According to a defined work flow, which in addition to troublesome phenotypic identification takes clinical relevance into account, 233 isolates were subjected to ITS sequence analysis. Sequencing resulted in successful identification for 78.6% of the analyzed isolates (57.1% at species level, 21.5% at genus level). In comparison, extended in-depth phenotypic characterization of the isolates subjected to sequencing achieved taxonomic assignment for 47.6% of these, with a mere 13.3% at species level. Optimization of DNA extraction further improved the efficacy of molecular identification. This study is the first of its kind to testify to the systematic implementation of sequence-based identification procedures in the routine workup of mold isolates in the diagnostic mycology laboratory. |
| Databáze: |
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