Identification of StaphylococcusSpecies Directly from Positive Blood Culture Broth by Use of Molecular and Conventional Methods

Autor: Mehta, Maitry S., Paule, Suzanne M., Thomson, Richard B., Kaul, Karen L., Peterson, Lance R.
Zdroj: Journal of Clinical Microbiology; April 2009, Vol. 47 Issue: 4 p1082-1086, 5p
Abstrakt: ABSTRACTWe compared two real-time PCR assays (both by the use of melting curve analysis) for their ability to identify Staphylococcusspecies directly from 200 positive blood culture bottles. The PCR assays correctly identified 83% to 94% of the Staphylococcusisolates to species clusters. Molecular testing significantly outperformed commercially available latex tests (sensitivity for both latex tests, <15%) when it was used directly with broth from signal-positive blood cultures.
Databáze: Supplemental Index