| Autor: |
Massung, Robert F., Levin, Michael L., Munderloh, Ulrike G., Silverman, David J., Lynch, Meghan J., Gaywee, Jariyanart K., Kurtti, Timothy J. |
| Zdroj: |
Journal of Clinical Microbiology; July 2007, Vol. 45 Issue: 7 p2138-2143, 6p |
| Abstrakt: |
ABSTRACTThe first tissue culture isolates of the unique Anaplasma phagocytophilumstrain, Ap-Variant 1, were obtained in the Ixodes scapularistick-derived cell line ISE6. Two isolates were from goat blood samples: one from a goat infected with I. scapularisticks from Rhode Island and a second from a goat infected by serial passage of blood from the first infected goat. Eight isolates were made directly from I. scapularisticks collected from white-tailed deer in Minnesota and represent the first isolations of an Anaplasmaspecies directly from ticks. Each of the 10 isolates had a 16S rRNA gene sequence identical to that previously described for Ap-Variant 1, but differences within the ankgene were found that suggest natural variation. Prevalence of Anaplasmain the Minnesota ticks was 63.9%; 23 of 36 ticks tested by PCR were positive. Six of the tick-derived isolates were obtained from a set of 18 PCR-positive ticks, for a 33.3% isolation success rate. The conservation of host tropism among the Rhode Island and Minnesota isolates of Ap-Variant 1 was examined by use of experimental infections of mice and a goat. A Minnesota tick-derived isolate (MN-61-2) was used to inoculate nai¨ve animals, and this isolate was able to infect a goat but unable to infect each of five mice, confirming that the Minnesota isolates have the same host tropism as Ap-Variant 1 from the northeastern United States. Light and electron microscopy of the Ap-Variant 1 isolate MN-61-2 in ISE6 cells showed cytoplasmic inclusions characteristic of A. phagocytophilumwith pleomorphic bacteria in membrane-bound vacuoles and both electron-dense and electron-lucent forms. |
| Databáze: |
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