Classifying spaTypes in Complexes Improves Interpretation of Typing Results for Methicillin-Resistant Staphylococcus aureus

Autor: Ruppitsch, Werner, Indra, Alexander, Sto¨ger, Anna, Mayer, Barbara, Stadlbauer, Silke, Wewalka, Gu¨nther, Allerberger, Franz
Zdroj: Journal of Clinical Microbiology; July 2006, Vol. 44 Issue: 7 p2442-2448, 7p
Abstrakt: ABSTRACTA total of 382 isolates of methicillin-resistant Staphylococcus aureusoriginating from three Austrian regions and one adjacent Italian region (Vienna, Lower Austria, North Tyrol, and South Tyrol) were typed by DNA sequence analysis of the variable repeat region of the protein A gene (spatyping). The strain collection consisted of arbitrarily chosen isolates originating from clinical specimens taken in the years 2003 to 2005 at 17 hospitals. The most common spatypes found were t001 (28.8% of all isolates), t190 (27.0%), t008 (14.1%), and t041 (11.3%). The 42 remaining spatypes accounted for =2.4% each. The dominating spatypes varied between the different regions. As short sequence DNA repeat units are unstable entities, the 46 spatypes were classified into seven spacomplexes with respect to short sequence repeat unit composition and organization. Such classification into complexes can provide additional information for the hospital epidemiologist, empowering one to differentiate the introduction of a new strain from mere variation of endemic spatypes.
Databáze: Supplemental Index