Development of a Triplex Real-Time PCR Assay for Detection of Panton-Valentine Leukocidin Toxin Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus
Autor: | McDonald, Ryan R., Antonishyn, Nick A., Hansen, Toni, Snook, Laelie A., Nagle, Evelyn, Mulvey, Michael R., Levett, Paul N., Horsman, Greg B. |
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Zdroj: | Journal of Clinical Microbiology; December 2005, Vol. 43 Issue: 12 p6147-6149, 3p |
Abstrakt: | ABSTRACTCommunity-associated methicillin-resistant Staphylococcus aureusharboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA. |
Databáze: | Supplemental Index |
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