| Autor: |
Mishra, A., Singhal, A., Chauhan, D. S., Katoch, V. M., Srivastava, K., Thakral, S. S., Bharadwaj, S. S., Sreenivas, V., Prasad, H. K. |
| Zdroj: |
Journal of Clinical Microbiology; November 2005, Vol. 43 Issue: 11 p5670-5678, 9p |
| Abstrakt: |
ABSTRACTMycobacterium tuberculosisand M. bovisinfect animals and humans. Their epidemiologies in developed and developing countries differ, owing to differences in the implementation of preventive measures (World Health Organization, 1999). Identification and differentiation of these closely related mycobacterial species would help to determine the source, reservoirs of infection, and disease burden due to diverse mycobacterial pathogens. The utility of the hupBgene (Rv2986c in M.?tuberculosis, or Mb3010cin M.?bovis) to differentiate M. tuberculosisand M. boviswas evaluated by a PCR-restriction fragment length polymorphism (RFLP) assay with 56 characterized bovine isolates (S. Prabhakar et al., J. Clin. Microbiol. 42:2724-2732, 2004). The degree of concordance between the PCR-RFLP assay and the microbiological characterization was 99.0% (P< 0.001). A nested PCR (N-PCR) assay was developed, replacing the PCR-RFLP assay for direct detection of M. tuberculosisand M. bovisin bovine samples. The N-PCR products of M. tuberculosisand M. boviscorresponded to 116 and 89 bp, respectively. The detection limit of mycobacterial DNA by N-PCR was 50 fg, equivalent to five tubercle bacilli. M. tuberculosisand/or M. boviswas detected in 55.5% (105/189) of the samples by N-PCR, compared to 9.4% (18/189) by culture. The sensitivities of N-PCR and culture were 97.3 and 29.7, respectively, and their specificities were 22.2 and 77.7%, respectively. The percentages of animals or samples identified as infected with M.?tuberculosisor M. bovisby N-PCR and culture reflected the clinical categorizations of the cattle (Pof <0.05 to <0.01). Mixed infection by N-PCR was detected in 22 animals, whereas by culture mixed infection was detected in 1 animal. |
| Databáze: |
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