| Autor: |
Sacchi, Claudio T., Alber, Dietmar, Dull, Peter, Mothershed, Elizabeth A., Whitney, Anne M., Barnett, Gwen A., Popovic, Tanja, Mayer, Leonard W. |
| Zdroj: |
Journal of Clinical Microbiology; August 2005, Vol. 43 Issue: 8 p3734-3742, 9p |
| Abstrakt: |
ABSTRACTA molecular typing method based on the 16S rRNA sequence diversity was developed for Haemophilus influenzaeisolates. A total of 330 H. influenzaeisolates were analyzed, representing a diverse collection of U.S. isolates. We found a high level of 16S rRNA sequence heterogeneity (up to 2.73%) and observed an exclusive correlation between 16S types and serotypes (a to f); no 16S type was found in more than one serotype. Similarly, no multilocus sequence typing (MLST) sequence type (ST) was found in more than one serotype. Our 16S typing and MLST results are in agreement with those of previous studies showing that serotypable H. influenzaeisolates behave as highly clonal populations and emphasize the lack of clonality of nontypable (NT) H. influenzaeisolates. There was not a 1:1 correlation between 16S types and STs, but all H. influenzaeserotypable isolates clustered similarly. This correlation was not observed for NT H. influenzae; the two methods clustered NT H. influenzaeisolates differently. 16S rRNA gene sequencing alone provides a level of discrimination similar to that obtained with the analysis of seven genes for MLST. We demonstrated that 16S typing is an additional and complementary approach to MLST, particularly for NT H. influenzaeisolates, and is potentially useful for outbreak investigation. |
| Databáze: |
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