| Autor: |
Streutker, C. J., Bernstein, C. N., Chan, V. L., Riddell, R. H., Croitoru, K. |
| Zdroj: |
Journal of Clinical Microbiology; February 2004, Vol. 42 Issue: 2 p660-664, 5p |
| Abstrakt: |
ABSTRACTThe inflammatory bowel diseases are considered an abnormal host immune response to an environmental stimulus. Evidence suggests a role for intestinal bacteria in initiating and/or providing an ongoing stimulus for inflammation in inflammatory bowel disease. Helicobacter pyloriis the major cause of active chronic gastritis and peptic ulcers in humans and has been linked to gastric carcinoma and lymphoma. Studies in various animal models, particularly mice, have identified enterohepatic Helicobacterspecies that are capable of causing hepatitis and enterocolitis. We hypothesize that Helicobacterspecies may have a role in maintaining inflammation in humans with inflammatory bowel disease. In order to investigate this, biopsy specimens were obtained from patients with and without inflammatory bowel disease. DNA was extracted from the tissues and subjected to PCR with primers designed to detect the ribosomal DNA of members of the Helicobacterspecies. DNA from six biopsy samples from 60 inflammatory bowel disease patients tested positive. This included 5 of 33 ulcerative colitis patients that were positive compared to 0 of 29 age-matched controls (P< 0.04). Sequencing of the bands produced by PCR amplification revealed =99% homology with H. pylori. These results indicate that a member of the Helicobacterspecies may be involved in some cases of ulcerative colitis. |
| Databáze: |
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