| Autor: |
Santos, Adriana, Queiroz, es, Me´nard, Armelle, Marais, Armelle, Rocha, Gifone Aguiar, Oliveira, Celso Affonso, Nogueira, Ana Margarida Miguel Ferreira, Uzeda, Milton, Me´graud, Francis |
| Zdroj: |
Journal of Clinical Microbiology; April 2003, Vol. 41 Issue: 4 p1651-1655, 5p |
| Abstrakt: |
ABSTRACTComparison of gastric carcinoma and gastritis isolates showed the presence of genes, probably carcinoma associated (JHP947and JHP940), that are situated in a Helicobacter pylorigenome region (45 kb in J99 and 68 kb in 26695) called the “plasticity region.” This region presents a great variability of DNA sequences. We investigated, by PCR, the presence of the JHP940and JHP947genes, as well as the presence of a third gene which seems to be associated with gastritis (HP986), on H. pyloristrains isolated from 200 Brazilian patients, 79 of whom had gastric carcinomas and 53 of whom had duodenal ulcers, to confirm this association. Gastritis isolates (n= 68) were included as a control. We also evaluated if these genes were related to the virulence-associated cagAgenotype. The present methodology did not permit definitive conclusions to be reached regarding the association between the JHP940gene and gastric carcinoma or between the HP986gene and gastritis. However, we showed that the JHP947gene might be implicated in the development of both duodenal ulcer and gastric carcinoma. The presence of the JHP947gene was associated with the cagA-positive genotype. The JHP947gene is a novel virulence marker candidate of H. pylori. |
| Databáze: |
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