| Autor: |
Schofield, David A., Westwater, Caroline, Paulling, Emily E., Nicholas, Peter J., Balish, Edward |
| Zdroj: |
Journal of Clinical Microbiology; February 2003, Vol. 41 Issue: 2 p831-834, 4p |
| Abstrakt: |
ABSTRACTHistopathology archives represent a vast source of infectious disease specimens that can be used to elucidate important disease processes. In this report, we describe a method to detect Candida albicansgene expression from infected, formalin-fixed, paraffin-embedded mouse tissue. By use of glass beads to break open the fungal cells and proteinase K treatment, RNA was extracted routinely from tissue sections that had been fixed for up to 72 h. Upon reverse transcription of the RNA and nested PCR, the procedure detected C. albicans“housekeeping” and putative virulence genes. |
| Databáze: |
Supplemental Index |
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