| Autor: |
Girardeau, Jean Pierre, Lalioui, Lila, Said, A. Mohamed Ou, De Champs, Christophe, Le Bougue´nec, Chantal |
| Zdroj: |
Journal of Clinical Microbiology; January 2003, Vol. 41 Issue: 1 p218-226, 9p |
| Abstrakt: |
ABSTRACTThe afimbrial AfaE-VIII adhesin is common among Escherichia coliisolates from calves with intestinal and/or extraintestinal infections and from humans with sepsis or pyelonephritis. The virulence genotypes of 77 Escherichia coli afa-8isolates from farm animals and humans were compared to determine whether any trait of commonality exists between isolates of the different host species. Over half of the extraintestinal afa-8isolates were associated with papand f17Acadhesin genes and contained virulence genes (pap, hly, and cnf1) which are characteristic of human extraintestinal pathogenic E. coli(ExPEC). PapG, which occurs as three known variants (variants I to III), is encoded by the corresponding three alleles of papG. Among the pap-positive strains, new papGvariants (papGrs) that differed from the isolates with genes for the three adhesin classes predominated over isolates with papGallele III, which in turn were more prevalent than those with allele II. The data showed the substantial prevalence of the enteroaggregative E. coliheat-stable enterotoxin gene (east1) among afa-8isolates. Most of the afa-8isolates harbored the high-pathogenicity island (HPI) present in pathogenic Yersinia; however, two-thirds of the HPI-positive strains shared a truncated HPI integrase gene. The presence of ExPEC-associated virulence factors (VFs) in extraintestinal isolates that carry genes typical of enteric strains and that express O antigens associated with intestinal E. coliis consistent with transfer of VFs and O-antigen determinants between ExPEC and enteric strains. The similarities between animal and human ExPEC strains support the hypothesis of overlapping populations, with members of certain clones or clonal groups including animal and human strains. The presence of multiple-antibiotic-resistant bovine afa-8strains among such clones may represent a potential public health risk. |
| Databáze: |
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