| Autor: |
Kidane, Dawit, Olobo, Joseph O., Habte, Abebe, Negesse, Yohannes, Aseffa, Abraham, Abate, Getahun, Yassin, Mohammed A., Bereda, Kiflu, Harboe, Morten |
| Zdroj: |
Journal of Clinical Microbiology; November 2002, Vol. 40 Issue: 11 p4230-4234, 5p |
| Abstrakt: |
ABSTRACTTuberculous lymphadenitis (TBLN) is a common form of extrapulmonary tuberculosis with multiple differential diagnoses. Demonstration of the etiologic agent by smear microscopy or culture of fine needle aspirate (FNA) specimens is often unsuccessful. FNA specimens from 40 patients presenting at a rural health center in South Ethiopia and diagnosed as positive for TBLN on the basis of clinical and cytological criteria were analyzed for mycobacterial DNA by PCR. Thirty (75%) had cervical lymphadenitis and 11 (27.5%) were seropositive for human immunodeficiency virus (HIV). Three primer sets were initially used to identify the causative agent at the genus (antigen 85 complex), complex (IS6110insertion sequence), and species (pncAgene and allelic variation) levels. Among the forty TBLN cases, 35 (87.5%) were positive by PCR at the genus and complex levels. Based on PCR for detection of allelic variation at position 169, 24 (68.6%) of the 35 were positive for Mycobacterium tuberculosisand 6 (17.1%) were positive for M. bovis. These six were positive in additional PCR assays using the JB21-JB22 primer set, which is highly specific for M. bovis.Five (14.1%) showed amplification for both M. tuberculosisand M. boviswith the allele-specific primer set.Cooccurrence of pyrazinamide (PZA)-sensitive and -resistant M. tuberculosisin those five cases was indicated, since all were negative in assays with the JB21-JB22 primer set. This feature was seen in 3 of 11 HIV-positive and 2 of 29 HIV-negative individuals (P< 0.001). Conclusion: among 35 PCR-positive cases of TBLN from southern Ethiopia, 29 (82.9%) were caused by M. tuberculosisand six (17.1%) were caused by M. bovis. |
| Databáze: |
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