| Autor: |
Niemann, S., Ru¨sch-Gerdes, S., Joloba, M. L., Whalen, C. C., Guwatudde, D., Ellner, J. J., Eisenach, K., Fumokong, N., Johnson, J. L., Aisu, T., Mugerwa, R. D., Okwera, A., Schwander, S. K. |
| Zdroj: |
Journal of Clinical Microbiology; September 2002, Vol. 40 Issue: 9 p3398-3405, 8p |
| Abstrakt: |
ABSTRACTThe population structure of 234 Mycobacterium tuberculosiscomplex strains obtained during 1995 and 1997 from tuberculosis patients living in Kampala, Uganda (East Africa), was analyzed by routine laboratory procedures, spoligotyping, and IS6110restriction fragment length polymorphism (RFLP) typing. According to biochemical test results, 157 isolates (67%) were classified as M. africanumsubtype II (resistant to thiophen-2-carboxylic acid hydrazide), 76 isolates (32%) were classified as M. tuberculosis, and 1 isolate was classified as classical M. bovis. Spoligotyping did not lead to clear differentiation of M. tuberculosisand M. africanum, but all M. africanumsubtype II isolates lacked spacers 33 to 36, differentiating them from M. africanumsubtype I. Moreover, spoligotyping was not sufficient for differentiation of isolates on the strain level, since 193 (82%) were grouped into clusters. In contrast, in the IS6110-based dendrogram, M. africanumstrains were clustered into two closely related strain families (Uganda I and II) and clearly separated from the M. tuberculosisisolates. A further characteristic of both M. africanumsubtype II families was the absence of spoligotype spacer 40. All strains of family I also lacked spacer 43. The clustering rate obtained by the combination of spoligotyping and RFLP IS6110analysis was similar for M. africanumand M. tuberculosis, as 46% and 49% of the respective isolates were grouped into clusters. The results presented demonstrate that M. africanumsubtype II isolates from Kampala, Uganda, belong to two closely related genotypes, which may represent unique phylogenetic branches within the M. tuberculosiscomplex. We conclude that M. africanumsubtype II is the main cause of human tuberculosis in Kampala, Uganda. |
| Databáze: |
Supplemental Index |
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