Real-Time PCR Detection of Salmonellain Suspect Foods from a Gastroenteritis Outbreak in Kerr County, Texas

Autor: Daum, Luke T., Barnes, William J., McAvin, James C., Neidert, Margaret S., Cooper, Lynn A., Huff, William B., Gaul, Linda, Riggins, W. S., Morris, Sandra, Salmen, Ann, Lohman, Kenton L.
Zdroj: Journal of Clinical Microbiology; August 2002, Vol. 40 Issue: 8 p3050-3052, 3p
Abstrakt: ABSTRACTIn June 2001, an outbreak of acute gastroenteritis among 109 attendees of a church picnic in Kerr County, Texas, was reported. A 5'-nuclease PCR assay was used to screen for Salmonellain nine food items from the buffet line. Barbeque chicken B tested positive for Salmonella, and no amplification was detected in the remaining food items. These PCR findings were consistent with culture results and were confirmed by direct nucleotide sequencing. Salmonella entericaserotype Panama was cultured from both food and patient stool samples.
Databáze: Supplemental Index