| Autor: |
Darwin, Ling H., Cullen, Allison P., Arthur, Patrick M., Long, Carole D., Smith, Kim R., Girdner, Jennifer L., Hook, Edward W., Quinn, Thomas C., Lorincz, Attila T. |
| Zdroj: |
Journal of Clinical Microbiology; February 2002, Vol. 40 Issue: 2 p641-644, 4p |
| Abstrakt: |
ABSTRACTDigene's Hybrid Capture 2 (HC2) CT/GC, CT-ID, and GC-ID DNA tests were evaluated by comparison to traditional culture methods for detecting Chlamydia trachomatisand Neisseria gonorrhoeaeinfections in 669 cervical specimens from high-risk female populations attending two sexually transmitted disease clinics. For detection of either or both infections, the HC2 CT/GC test algorithm had 93.8% sensitivity and 95.9% specificity compared to those of culture. After resolution of discrepant results by direct fluorescent-antibody (DFA) staining or PCR assay, the relative sensitivity and specificity of the HC2 CT/GC test algorithm increased to 94.8 and 99.8%, while the values for culture were 83.6% (McNemar's Pvalue, 0.0062) and 100%, respectively. For detection of the individual pathogens, the relative sensitivities for the HC2 CT-ID and GC-ID tests were 97.2 and 92.2% and the specificities were greater than 99% compared to culture adjucated by DFA staining and PCR. Test performance varied at the two clinics: the HC2 CT/GC algorithm, CT-ID, and GC-ID tests had significantly higher sensitivities (McNemar's Pvalue, <0.05) than that of culture for the population at one clinic as well as for the combined populations. At the other clinic, the HC2 tests performed as well as culture. |
| Databáze: |
Supplemental Index |
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