Genomic and Phylogenetic Analysis of Hepatitis C Virus Isolates from Argentine Patients: a Six-Year Retrospective Study

Autor: Quarleri, J. F., Robertson, B. H., Mathet, V. L., Feld, M., Espi´nola, L., Requeijo, M. P., Mando´, O., Carballal, G., a
Zdroj: Journal of Clinical Microbiology; December 2000, Vol. 38 Issue: 12 p4560-4568, 9p
Abstrakt: ABSTRACTTyping of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23.0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogenetic relatedness among sequences from both 5' untranslated region (n= 50) and nonstructural 5B coding region (n= 15) was established.
Databáze: Supplemental Index