Application of tRNA Intergenic Spacer PCR for Identification of EnterococcusSpecies

Autor: Baele, Margo, Baele, Paul, Vaneechoutte, Mario, Storms, Virginie, Butaye, Patrick, Devriese, Luc A., Verschraegen, Gerda, Gillis, Monique, Haesebrouck, Freddy
Zdroj: Journal of Clinical Microbiology; November 2000, Vol. 38 Issue: 11 p4201-4207, 7p
Abstrakt: ABSTRACTtRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faeciumshowed minor but reproducible differences with Enterococcus durans, while Enterococcus hiraewas easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosusgenerated highly similar though distinctive patterns.
Databáze: Supplemental Index