| Autor: |
Escudero, Raquel, Barral, Marta, Pe´rez, Azucena, Vitutia, M. Mar, Garci´a-Pe´rez, Ana L., Jime´nez, Santos, Sellek, Ricela E., Anda, Pedro |
| Zdroj: |
Journal of Clinical Microbiology; November 2000, Vol. 38 Issue: 11 p4026-4033, 8p |
| Abstrakt: |
ABSTRACTFifteen Borrelia burgdorferisensu lato isolates from questing ticks and skin biopsy specimens from erythema migrans patients in three different areas of Spain were characterized. Four different genospecies were found (nine Borrelia garinii, including the two human isolates, three B. burgdorferisensu stricto, two B. valaisiana, and one B. lusitaniae), showing a diverse spectrum of B. burgdorferisensu lato species. B. gariniiisolates were highly variable in terms of pulsed-field gel electrophoresis pattern and OspA serotype, with four of the seven serotypes described. One of the human isolates was OspA serotype 5, the same found in four of seven tick isolates. The second human isolate was OspA serotype 3, which was not present in ticks from the same area. Seven B. gariniiisolates were able to disseminate through the skin of C3H/HeN mice and to cause severe inflammation of joints. One of the two B. valaisianaisolates also caused disease in mice. Only one B. burgdorferisensu stricto isolate was recovered from the urinary bladder. One isolate each of B. valaisianaand B. lusitaniaewere not able to disseminate through the skin of mice or to infect internal organs. In summary, there is substantial diversity in the species and in the pathogenicity of B. burgdorferisensu lato in areas in northern Spain where Lyme disease is endemic. |
| Databáze: |
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