| Autor: |
Boyd, D. A., Conly, J., Dedier, H., Peters, G., Robertson, L., Slater, E., Mulvey, M. R. |
| Zdroj: |
Journal of Clinical Microbiology; June 2000, Vol. 38 Issue: 6 p2392-2394, 3p |
| Abstrakt: |
ABSTRACTA single vanD-containing Enterococcus faeciumstrain (N97-330) was isolated in Canada. ThevanD-containing region was cloned and sequenced. Although the proteins have more than 96% identity to a previously described vanDregion in BM4339, thevanSDgene contains a frameshift mutation that leads to a predicted truncated protein. Furthermore, sequence analysis of the ddlgene revealed the presence of an IS982-like element (ISEfm1) which interrupted the d-Ala–d-Ala ligase. This suggested the constitutive expression of the vanDoperon, which was confirmed. Pulsed-field gel electrophoresis fingerprinting demonstrated that BM4339 was not related to N97-330 (>15 band differences). Both strains contained multiple copies of the IS982-like element. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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