| Abstrakt: |
ABSTRACTWe evaluated three rapid identification systems—The Biomerieux rapid ID 32 STREP (ID32), the BBL Crystal rapid gram-positive identification (Crystal), and the Remel IDS RapID STR (IDS) systems—for their ability to identify 7 strains of Alloiococcus otitidis, 27 strains of Dolosigranulum pigrum, 3 strains of Ignavigranum ruoffiae, and 18 strains of 4 different Facklamiaspecies. Since none of these six species of gram-positive cocci are included in the identification databases for these systems, the correct identification for the strains tested should be “unacceptable ID” for the ID32 and Crystal systems or “no choice” for the IDS system. The ID32 system identified all 27 strains of D. pigrum, 6 of 18 Facklamiaspecies, and 2 of 3 cultures of I. ruoffiaeas “unacceptable ID.” The Crystal system identified 10 of 27 D. pigrum, 2 of 18 Facklamiaspecies, and 2 of 3I. ruoffiaestrains as “unacceptable ID.” The IDS system identified only 1 culture of D. pigrumas “no choice,” but it also identified 2 cultures of D. pigrumas a “questionable microcode” and 19 cultures of D. pigrumas an “inadequate ID, E. faecalis90%,S. intermedius9%.” A total of 2 of the 18 cultures ofFacklamiaand all 3 of the I. ruoffiaecultures were correctly identified as “no choice.” The most common misidentifications of Facklamiaspecies by the ID32 and IDS systems were as various Streptococcusspecies and asGemellaspecies. In the Crystal system, the most common erroneous identification was Micrococcus luteus. These data indicate the need for the commercial manufacturers of these products to update their databases to include newly described species of gram-positive cocci. |
