| Autor: |
White, Irene E., Campbell, Thomas B. |
| Zdroj: |
Journal of Clinical Microbiology; May 2000, Vol. 38 Issue: 5 p1992-1995, 4p |
| Abstrakt: |
ABSTRACTA real-time PCR assay for quantitation of Kaposi's sarcoma-associated herpes virus (KSHV or human herpesvirus 8) DNA was evaluated. The linear dynamic range was 10 to 105copies of KSHV DNA (r2> 0.99). The accuracy of DNA purification and quantitation was less than ±0.4 log10copies for samples that contained from 10 to 105copies of KSHV DNA. Cell-associated KSHV DNA was quantitated over a range of infected cell frequencies from 0.1 to 10-5, and cell-free KSHV DNA in plasma was quantitated over a range of 100 to 106copies/ml. Real-time PCR provides a convenient method for quantitation of cell-free and cell-associated KSHV DNA in laboratory and clinical specimens. |
| Databáze: |
Supplemental Index |
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