| Autor: |
Hudson, Charlene R., Quist, Charlotte, Lee, Margie D., Keyes, Kathleen, Dodson, Sara V., Morales, Cesar, Sanchez, Susan, White, David G., Maurer, John J. |
| Zdroj: |
Journal of Clinical Microbiology; May 2000, Vol. 38 Issue: 5 p1860-1865, 6p |
| Abstrakt: |
ABSTRACTSalmonellainfections have been implicated in large-scale die-offs of wild birds in the United States. Although we know quite a bit about the epidemiology of Salmonellainfection among domestic fowl, we know little about the incidence, epidemiology, and genetic relatedness of salmonellae in nondomestic birds. To gain further insight into salmonellae in these hosts, 22Salmonellaisolates from diseased nondomestic birds were screened for the presence of virulence and antibiotic resistance-associated genes and compared genetically using pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA analysis. Of the 22 Salmonellaisolates examined, 15 were positive for the invasion gene invAand the virulence plasmid-associated genes spvCand pef. Most (15 of 22) were generally sensitive to antibiotics. However, twoSalmonellaisolates from pet birds were identified asSalmonella entericaserovar Typhimurium DT104. Despite the general susceptibility of these Salmonellaisolates to most antimicrobial agents, antibiotic resistance-associated genesintI1, merA, and aadA1were identified in a number of these isolates. Five distinctXbaI and nine distinct BlnI DNA patterns were observed for the 22 Salmonellaisolates typed by PFGE. PFGE analysis determined that Salmonellaisolates from passerines in Georgia and Wyoming were genetically related. |
| Databáze: |
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