| Autor: |
Field, Peter R., Mitchell, Jody L., Santiago, Avelina, Dickeson, David J., Chan, Sau-Wan, Ho, David W. T., Murphy, Alan M., Cuzzubbo, Andrea J., Devine, Peter L. |
| Zdroj: |
Journal of Clinical Microbiology; April 2000, Vol. 38 Issue: 4 p1645-1647, 3p |
| Abstrakt: |
ABSTRACTA commercially available enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Q fever (PanBio Coxiella burnetiiimmunoglobulin M [IgM] ELISA, QFM-200) was compared to the indirect fluorescent antibody test (IFAT) for C. burnetiiIgM and the complement fixation test (CFT). The ELISA demonstrated 92% agreement with the reference method (IFAT), and gave a sensitivity of 99% (69 of 70 samples) and a specificity of 88% (106 of 121). Specificity can be increased with confirmation by IFAT. CFT was found to have a specificity of 90% (107 of 119), although it was lacking in sensitivity (73%; 51 of 70). No cross-reactivity was observed in the ELISA with serum samples from patients with mycoplasma (n= 6), chlamydia (n= 5), or legionella (n= 4) infections, although 2 of 5 patients with leptospirosis and 1 of 4 samples containing rheumatoid factor (RF) demonstrated positive results in the ELISA. Results indicate that the performance of the PanBio C. burnetii(Q fever) IgM ELISA (F= 187) is superior to that of CFT (F= 163), and consequently the ELISA should be a useful aid in the diagnosis of acute Q fever. |
| Databáze: |
Supplemental Index |
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