| Autor: |
Gales, A. C., Pfaller, M. A., Houston, A. K., Joly, S., Sullivan, D. J., Coleman, D. C., Soll, D. R. |
| Zdroj: |
Journal of Clinical Microbiology; December 1999, Vol. 37 Issue: 12 p3804-3808, 5p |
| Abstrakt: |
ABSTRACTTo have a better understanding of the role of Candida dubliniensisin clinical infections, it is essential that microbiology laboratories can identify this species rapidly and accurately in clinical specimens. C. dubliniensishas been reported to lack the ability to utilize xylose (XYL) and a-methyl-d-glucoside (MDG) and to grow poorly or not at all at 45°C, whereas Candida albicansisolates utilize XYL and MDG and usually grow well at 45°C. We tested 66 isolates ofC. dubliniensisand 100 isolates of C. albicanswith both the API 20C AUX and Vitek YBC systems to evaluate the ability of the XYL and MDG tests contained within each of these systems to distinguish between the two species. The ability to grow at 45°C was also examined. None of the C. dubliniensisisolates grew at 45°C, and 23 of 100 C. albicansisolates (23%) exhibited poor or no growth at 45°C. The XYL and MDG tests contained within the API 20C AUX system were both negative for all 66 C. dubliniensisisolates and were positive for 98 (XYL) and 56 (MDG) of the 100 C. albicansisolates. With the Vitek system, 64 of 66 C. dubliniensisisolates (97.0%) were XYL negative and 63 (95.0%) were MDG negative. Conversely, 96 of 100 C. albicansisolates (96.0%) were XYL positive and 100 (100.0%) were MDG positive with the Vitek system. Clinical microbiology laboratories could use lack of growth at 45°C and a negative XYL test with either the API 20C AUX or Vitek yeast identification system to provide a presumptive identification of C. dubliniensis. A negative MDG test result with either system would also be helpful but may misclassify C. albicansas C. dubliniensis, especially when the API 20C AUX system is used. |
| Databáze: |
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