| Autor: |
Loubinoux, Julien, Lozniewski, Alain, Lion, Christine, Garin, Daniel, Weber, Miche`le, Le Faou, Alain E. |
| Zdroj: |
Journal of Clinical Microbiology; August 1999, Vol. 37 Issue: 8 p2488-2492, 5p |
| Abstrakt: |
ABSTRACTFifty-six Pasteurella multocidastrains (40 P. multocidasubsp. septicaand 16 P. multocidasubsp. multocidastrains) isolated from the mouths of 56 dogs among the 134 living in a French canine military training center (132e Groupe Cynophile de l’Arme´e de Terre, Suippes, France) were studied by use of enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and restriction fragment length polymorphism (RFLP) techniques. Both techniques showed genomic heterogeneity of the strains studied. However, RFLP was more discriminatory than ERIC-PCR for differentiating P. multocidastrains. All but three pairs of strains were discriminated by RFLP, suggesting a limited circulation of strains between these dogs living in proximity. Although ERIC-PCR is easier and faster to perform, it cannot be recommended for epidemiological studies of P. multocidastrains. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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