| Autor: |
Badak, F. Zuhre, Goksel, Servet, Sertoz, Ruchan, Nafile, Bedii, Ermertcan, Safak, Cavusoglu, Cengiz, Bilgic, Altinay |
| Zdroj: |
Journal of Clinical Microbiology; May 1999, Vol. 37 Issue: 5 p1602-1605, 4p |
| Abstrakt: |
ABSTRACTThe feasibility of using nucleic acid probes directly from positive MB/BacT broth to identify mycobacteria was determined in this study. A total number of 2,727 specimens were cultured into the MB/BacT (Organon Teknika) automated system and on conventional Loweinstein-Jensen (LJ) slants. The Gen-Probe AccuProbe culture identification tests (DNA probes) were used on samples from bottles which were identified as positive for mycobacteria by MB/BacT. Samples of positive MB/BacT broth (0.1 ml) were used directly in the broth culture method for the DNA probes as published by Gen-Probe. Centrifugation of the contents of the bottle was not done prior to probe testing. The number of mycobacteria detected by MB/BacT and LJ was 253 (221 isolates of M. tuberculosisand 32 isolates of mycobacteria other than M. tuberculosis[MOTT]). A total of 96.4% (213 of 221) of the bottles growing M. tuberculosisproduced a positive direct DNA probe result for M. tuberculosiscomplex. One hundred percent (16 of 16) of the bottles growing M. gordonaeproduced a positive direct DNA probe result for M. gordonae. A total of 3.6% (8 of 221) of the bottles growingM. tuberculosisdid not yield a positive direct DNA probe result for M. tuberculosiscomplex. The testing of subcultures made onto solid media from the positive bottles by AccuProbe identified six of these eight M. tuberculosisisolates. Two (0.9%) M. tuberculosisisolates gave a negative result for the M. tuberculosisprobe test applied on the MB/BacT broth and its subculture. The rest of the positive MB/BacT bottles growing MOTT (16 of 32) were negative for M. gordonae, M. avium, M. intracellulare, and M. kansasiiprobes. The sensitivity and specificity of AccuProbe for the identification of M. tuberculosisandM. gordonaedirectly from MB/BacT broth were 96.4 and 100% for M. tuberculosisand 100 and 100% for M. gordonae, respectively. The direct testing of positive MB/BacT broth by AccuProbe, without prior centrifugation, allows for the accurate and rapid identification of M. tuberculosisandM. gordonae. |
| Databáze: |
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