Correlation between Presence of Viable Bacteria and Presence of Endotoxin in Middle-Ear Effusions

Autor: Dingman, Jeffrey R., Rayner, Mark G., Mishra, Suman, Zhang, Yingze, Ehrlich, Miles D., Post, J. Christopher, Ehrlich, Garth D.
Zdroj: Journal of Clinical Microbiology; November 1998, Vol. 36 Issue: 11 p3417-3419, 3p
Abstrakt: ABSTRACTThe presence of endotoxin (detected by the Limulusamebocyte lysate assay) was compared to the presence of viableHaemophilus influenzaeand Moraxella catarrhalis(detected by PCR) in 106 middle-ear effusions from pediatric patients with chronic otitis media. Endotoxin was found in 81 of the 106 specimens. Of these 81 specimens, 66 (81.5%) also tested positive for one or both of the gram-negative bacteria H. influenzaeand M. catarrhalis. The data suggest that viable gram-negative bacteria, detectable by PCR but often undetectable by culture, may be the source of endotoxin in middle-ear effusions.
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