| Autor: |
Valassina, Marcello, Soldateschi, Dario, Dal Maso, Grazia Maria, Santini, Laura, Bianchi, Silvia, Valensin, Pier Egisto, Cusi, Maria Grazia |
| Zdroj: |
Journal of Clinical Microbiology; November 1998, Vol. 36 Issue: 11 p3170-3172, 3p |
| Abstrakt: |
ABSTRACTThe nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coliby using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA). The recombinant antigen was reactive with positive human sera, and the reactivity correlated very well (r= 0.9) with that of a whole-virus antigen when tested by EIA with 30 TOS virus-positive and 30 TOS virus-negative serum samples. The results demonstrate that the recombinant N protein can be easily produced in a procaryotic system and used for diagnostic assays for TOS virus immunity. |
| Databáze: |
Supplemental Index |
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