| Autor: |
de los Monteros, Luz Elena Espinosa, Gala´n, Juan Carlos, Gutie´rrez, Montserrat, Samper, Sofi´a, Garci´a Mari´n, Juan F., Marti´n, Carlos, Domi´nguez, Lucas, de Rafael, Luis, Baquero, Fernando, Go´mez-Mampaso, Enrique, Bla´zquez, Jesu´s |
| Zdroj: |
Journal of Clinical Microbiology; January 1998, Vol. 36 Issue: 1 p239-242, 4p |
| Abstrakt: |
ABSTRACTAn allele-specific amplification method based on two genetic polymorphisms to differentiate Mycobacterium tuberculosisfrom Mycobacterium boviswas tested. Based on the differences found at position 169 in the pncAgenes fromM. tuberculosisand M. bovis, a PCR system which was able to differentiate most of the 237 M. tuberculosiscomplex isolates tested in one of the two species was developed. All 121 M. tuberculosisstrains showed the expected base (cytosine) at position 169. Most of the M. bovisisolates had a guanine at the cited position. Nevertheless, 18 of the 116 M. bovisisolates, all of them goat isolates, showed the pncApolymorphism specific to M. tuberculosis. These results suggest that goat M. bovismay be the nicotinamidase-missing link at the origin of theM. tuberculosisspecies. Based on the polymorphism found at position 285 in the oxyRgene, the same system was used to differentiate M. tuberculosisfrom M. bovis. In this case, DNAs from all 121 M. tuberculosisisolates had the expected base (guanine) at this position. In addition, all 116M. bovisisolates, including those from goats, showed the identical polymorphism (adenine). The oxyRallele-specific amplification method can differentiate M. bovisfromM. tuberculosis, is rapid (results can be obtained in less than 3 h), and is easy to perform. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
