PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes

Autor: Saunders, N A, Hallas, G, Gaworzewska, E T, Metherell, L, Efstratiou, A, Hookey, J V, George, R C
Zdroj: Journal of Clinical Microbiology; October 1997, Vol. 35 Issue: 10 p2689-2691, 3p
Abstrakt: A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types that were not included in the panel of capture probes were emm genotyped by sequencing.
Databáze: Supplemental Index