| Autor: |
Umemura, Masayuki, Wajjwalku, Worawidh, Upragarin, Narin, Liu, Tie, Nishimura, Hitoshi, Matsuguchi, Tetsuya, Nishiyama, Yukihiro, Wilson, Gary M., Yoshikai, Yasunobu |
| Zdroj: |
The Journal of Virology; September 2000, Vol. 74 Issue: 18 p8226-8233, 8p |
| Abstrakt: |
ABSTRACTTo investigate whether superantigen (SAG) from endogenous mouse mammary tumor virus functions as an immunogenic or a tumorigenic factor in tumor development, the BALB/c myeloma cell line FO was transfected with the SAG gene from the 3' Mtv-50long terminal repeat (LTR) open reading frame (ORF), the product of which was specific for Vß6. All five transfectants expressing Mtv-50LTR ORF mRNA showed stimulatory activity for Vß6 T-cell hybridomas in vitro; this activity was inhibited by the addition of anti-Mtv-7monoclonal antibody (MAb) or anti-major histocompatibility complex class II I-Adand I-EdMAb. All transfectants with the SAG gene grew more rapidly than did mock transfectants in BALB/c mice after subcutaneous inoculation, whereas all clones, including mock transfectants, grew equally well in athymic nude mice. A significant fraction of Vß6 T cells selectively expressed activation markers, including CD44high, CD62Llow, and CD69high, and produced large amounts of interleukin 5 (IL-5) and IL-6 in BALB/c mice inoculated with transfectants. These results suggested that the expression of viral SAG enhances the tumorigenicity of a myeloma cell line through the stimulation of SAG-reactive T cells. |
| Databáze: |
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