| Autor: |
He, Xiaodun, Lehman, I. R. |
| Zdroj: |
The Journal of Virology; June 2000, Vol. 74 Issue: 12 p5726-5728, 3p |
| Abstrakt: |
ABSTRACTA herpes simplex virus type 1 (HSV-1) OriSanalogue in which the A+T sequence linking the box I and II elements was replaced by two single-stranded oligo(dT)s is unwound by the UL9 protein-ICP8 complex. Unwinding of wild-type OriSby the UL9 protein-ICP8 complex was also observed under conditions which destabilize the A+T sequence. These experiments support a model for the unwinding of OriSin which destabilization of the A+T sequence can generate a single-stranded DNA binding site for ICP8, which then associates with the UL9 protein bound to boxes I and II to promote the bidirectional unwinding of OriS. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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