| Autor: |
Chen, D, Zeng, C Q, Wentz, M J, Gorziglia, M, Estes, M K, Ramig, R F |
| Zdroj: |
The Journal of Virology; November 1994, Vol. 68 Issue: 11 p7030-7039, 10p |
| Abstrakt: |
A template-dependent, in vitro rotavirus RNA replication system was established. The system initiated and synthesized full-length double-stranded RNAs on rotavirus positive-sense template RNAs. Native rotavirus mRNAs or in vitro transcripts, with bona fide 3' and 5' termini, derived from rotavirus cDNAs functioned as templates. Replicase activity was associated with a subviral particle containing VP1, VP2, and VP3 and was derived from native virions or baculovirus coexpression of rotavirus genes. A cis-acting signal involved in replication was localized within the 26 3'-terminal nucleotides of a reporter template RNA. Various biochemical and biophysical parameters affecting the efficiency of replication were examined to optimize the replication system. A replication system capable of in vitro initiation has not been previously described for Reoviridae. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
