Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood

Autor: Schwarz, T F, Serke, S, Hottenträger, B, von Brunn, A, Baurmann, H, Kirsch, A, Stolz, W, Huhn, D, Deinhardt, F, Roggendorf, M
Zdroj: The Journal of Virology; February 1992, Vol. 66 Issue: 2 p1273-1276, 4p
Abstrakt: Erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus B19. B19 virus DNA replication was highest 48 to 72 h after infection, and maximum levels of B19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. B19 virus propagated in vitro was infectious. This cell culture system with peripheral blood cells facilitates studies in vitro of B19 virus replication.
Databáze: Supplemental Index