Autor: |
Bathurst, I C, Chester, N, Gibson, H L, Dennis, A F, Steimer, K S, Barr, P J |
Zdroj: |
The Journal of Virology; July 1989, Vol. 63 Issue: 7 p3176-3179, 4p |
Abstrakt: |
A semisynthetic gene precisely encoding the 502 amino acids of the human immunodeficiency virus type 1 gag precursor (Pr53gag) was expressed in the yeast Saccharomyces cerevisiae. Amino acid sequence analysis of the recombinant Pr53gag showed that the amino terminus was fully blocked. Labeling of Pr53gag with [3H]myristic acid demonstrated that, as with Pr53gag isolated from virus-infected cells, the yeast-derived protein was demethionylated and N myristylated on glycine, the second amino acid residue. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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