| Autor: |
Auborn, K J, Markowitz, R B, Wang, E, Yu, Y T, Prives, C |
| Zdroj: |
The Journal of Virology; June 1988, Vol. 62 Issue: 6 p2204-2208, 5p |
| Abstrakt: |
Simian virus 40 T antigen has been shown previously to bind specifically with high affinity to sites within the regulatory region of double-stranded simian virus 40 DNA. Using competition filter binding and the DNA-binding immunoassay, we show that T antigen did not bind specifically to either early or late single-stranded DNA containing these binding sites. Moreover, T antigen did not bind these sequences present in single-stranded RNA, RNA/RNA duplexes, or RNA/DNA hybrids. T antigen did, however, bind as efficiently to single-stranded DNA-cellulose as to double-stranded DNA-cellulose. This binding was nonspecific because it was independent of the presence of T-antigen-binding sites. The implications of these observations are discussed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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