| Autor: |
Hewlett, M J, Axelrod, J H, Antinoro, N, Feld, R |
| Zdroj: |
The Journal of Virology; March 1982, Vol. 41 Issue: 3 p1089-1094, 6p |
| Abstrakt: |
We isolated six temperature-sensitive mutants of poliovirus type 1 (Mahoney) by hydroxylamine mutagenesis and replica plating at 31, 33 (permissive), and 39 degrees C (restrictive). One of these mutants, designated tsB9, was chosen for more detailed examination. tsB9 accumulated 25% of the wild-type amount of virus-specific RNA at the restrictive temperature. We found that tsB9 was not able to synthesize mature, 35S single-stranded RNA at the restrictive temperature. In spite of the absence of significant RNA synthesis, tsB9 retained the ability to inhibit host protein synthesis during infection at 39 degrees C at about the same rate as wild-type virus. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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