| Autor: |
Dahlberg, J. E., Sawyer, R. C., Taylor, J. M., Faras, A. J., Levinson, W. E., Goodman, H. M., Bishop, J. M. |
| Zdroj: |
The Journal of Virology; May 1974, Vol. 13 Issue: 5 p1126-1133, 8p |
| Abstrakt: |
The enzymatic transcription of DNA from the 70SRNA of Rous sarcoma virus (RSV) is initiated on the 3' terminus of a molecule of 4SRNA which is hydrogen bonded to the viral genome. We labeled this primer with radioactive deoxynucleotides, and demonstrated that its release from 70SRNA by thermal denaturation was accompanied by a reduction in the template activity of the viral RNA. Two-dimensional electrophoresis in polyacrylamide gels separated the 4SRNAs associated with the 70SRNA of RSV into approximately eight fractions, each of which appeared to contain a discrete species of tRNA. The RNA in one of these fractions served as the principal primer for initiation of DNA synthesis by both detergent-disrupted virions of RSV and purified RNA-directed DNA polymerase with RSV 70SRNA as template. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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