| Autor: |
Henry, Patrick H., Schnipper, Lowell E., Samaha, Richard J., Crumpacker, Clyde S., Lewis, Andrew M., Levine, Arthur S. |
| Zdroj: |
The Journal of Virology; May 1973, Vol. 11 Issue: 5 p665-671, 7p |
| Abstrakt: |
Certain biophysical characteristics of the DNA from each of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses (Ad2+ND1, Ad2+ND2, Ad2+ND3, Ad2+ND4, Ad2+ND5) have been determined. The guanine plus cytosine content varied from 55 to 57% and was not significantly different from that of nonhybrid Ad2 (56%), and the hybrid DNA molecules had mean molecular lengths which were similar to that of the standard, Ad2. The Ad2 and SV40 components of each hybrid were linked by alkali-resistant, presumably covalent bonds. The percentage of SV40 DNA in each hybrid virus was determined by hybridization with SV40 complementary RNA in a calibrated system. The results indicate that each hybrid virus DNA contains a different percentage of SV40 nucleotide sequences. The estimated size of the SV40 DNA component varies from 48,000 daltons for Ad2+ND3to 840,000 daltons for Ad2+ND4, the latter being equivalent to between one-fourth and one-third of the SV40 genome. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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