| Autor: |
Armentrout, Richard W., Skoog, Lambert, Rutberg, Lars |
| Zdroj: |
The Journal of Virology; March 1971, Vol. 7 Issue: 3 p359-371, 13p |
| Abstrakt: |
The effects of Escherichia coliexonuclease I, exonuclease III, and deoxyribonucleic acid (DNA) polymerase on the biological activity of mature DNA from temperate Bacillusbacteriophage f105 were investigated. Intact DNA loses infectivity rapidly upon exposure to exonuclease III. Although there is an overall decrease in marker rescue from exonuclease III-digested DNA, digestion preferentially affects markers at the end of the genetic map. This is taken to indicate a nonpermuted gene sequence in mature DNA. Incubation of mature DNA in the presence of exonuclease I or DNA polymerase has no effect on its biological activity. The possible structure of the ends of mature f105 DNA is discussed. The rate of digestion of mature f105 DNA by exonuclease III is only about 1/20 the rate of ? DNA. Results of digestion of various DNA substrates by exonuclease III indicate that the enzyme distinguishes between different DNA terminal structures. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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