| Autor: |
Carlone, G M, Thomas, M L, Rumschlag, H S, Sottnek, F O |
| Zdroj: |
Journal of Clinical Microbiology; September 1986, Vol. 24 Issue: 3 p330-332, 3p |
| Abstrakt: |
A rapid microprocedure for isolating detergent (sodium N-lauroyl sarcosinate)-insoluble major outer membrane proteins from Haemophilus species produced results qualitatively identical to those obtained with a commonly used preparative isolation procedure. Proteins isolated by both procedures were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after staining with Coomassie brilliant blue R-250. The time for outer membrane protein isolation was substantially reduced with the rapid procedure, allowing a larger number of membrane preparations to be obtained rapidly for routine analysis. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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