| Autor: |
Rotbart, H A, Sawyer, M H, Fast, S, Lewinski, C, Murphy, N, Keyser, E F, Spadoro, J, Kao, S Y, Loeffelholz, M |
| Zdroj: |
Journal of Clinical Microbiology; October 1994, Vol. 32 Issue: 10 p2590-2592, 3p |
| Abstrakt: |
A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
