Diagnosis of human immunodeficiency virus (HIV) infection: multicenter evaluation of a newly developed anti-HIV 1 and 2 enzyme immunoassay

Autor: Hess, G, Avillez, F, Lourenco, M H, D'Agostino, F, Cambie, G, Piot, P, Vercauteren, G, Michl, U, Melchior, W, Bayer, H
Zdroj: Journal of Clinical Microbiology; February 1994, Vol. 32 Issue: 2 p403-406, 4p
Abstrakt: A new anti-human immunodeficiency virus type 1 and 2 (anti-HIV 1 and 2) test is described. It uses recombinant p24 and peptides covering gp32, gp41, and gp120 to identify HIV-1 and HIV-2 infections. This test has been shown to be specific (99.5%) and sensitive (99.8%). In this respect, the assay was equal or superior to anti-HIV 1 and 2 tests run as references. The test was able to discriminate sera from patients with HIV infections from those from uninfected individuals with excellence; it also exerted high intra- and interassay precisions. The "modular" concept of the test allows the use of single components (gp32 or gp41) to separate between HIV-2 and HIV-1 infections, respectively.
Databáze: Supplemental Index