| Autor: |
Perlmann, H K, Berzins, K, Wåhlin, B, Udomsangpetch, R, Ruangjirachuporn, W, Wahlgren, M, Perlmann, P H |
| Zdroj: |
Journal of Clinical Microbiology; December 1987, Vol. 25 Issue: 12 p2347-2354, 8p |
| Abstrakt: |
Pf155 is a merozoite-derived polypeptide antigen which the parasite Plasmodium falciparum deposits in the membranes of erythrocytes at invasion. Eleven laboratory strains or clones of P. falciparum and a large number of isolates obtained from patients from different parts of the world were studied for antigenic diversity in Pf155. Immunoglobulin G antibodies from different serum samples from P. falciparum-infected donors were affinity purified on monolayers of glutaraldehyde-fixed and air-dried erythrocytes infected with P. falciparum of different origins and tested in different combinations by immunoblotting, reinvasion inhibition, and a modified immunofluorescence procedure in which the membranes of recently infected erythrocytes were stained. Similar experiments were performed with monoclonal and oligoclonal antibodies specific for different epitopes in the C-terminal region of Pf155. No strain- or isolate-associated antigenic diversity or size variation of Pf155 was detected, indicating that the immunodominant regions of this antigen are highly conserved throughout the world. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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