| Autor: |
Panda, C S, Riley, L W, Kumari, S N, Khanna, K K, Prakash, K |
| Zdroj: |
Journal of Clinical Microbiology; September 1990, Vol. 28 Issue: 9 p2122-2124, 3p |
| Abstrakt: |
We compared an alkaline phosphatase-conjugated oligonucleotide DNA probe with the Sereny test to determine the sensitivity and specificity of the probe in detecting virulent Shigella strains. The probe hybridized with all 52 Sereny-test-positive strains (sensitivity, 100%) and 4 of 21 Sereny-test-negative strains (specificity, 81%). The probe did not hybridize with any of the Sereny-test-negative S. dysenteriae type 1 strains. This nonradioactive, synthetic probe provides a simple, rapid way to test a large number of strains simultaneously in a field setting, which will contribute to an improved understanding of the epidemiologic patterns of shigellosis in developing countries. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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