| Autor: |
Khabbaz, R F, Standiford, H C, Bernstein, D, Nipper, H C, Tatem, B A, Smalls, U, Drusano, G L, Caplan, E |
| Zdroj: |
Journal of Clinical Microbiology; November 1985, Vol. 22 Issue: 5 p699-701, 3p |
| Abstrakt: |
Levels of amikacin in serum were determined in 106 serum specimens by a latex agglutination inhibition card test and by radioimmunoassay (RIA). Linear regression analysis demonstrated a high degree of correlation between the two assays (latex = 0.95 (RIA) + 0.69; r = 0.97). Assay of three control sera containing 7.5, 15, and 30 micrograms of amikacin per ml on 7 separate days showed good reproducibility with a coefficient of variation of 0 to 11.7% for the latex assay compared with 7.01 to 22.2% for RIA. Recovery of amikacin in spiked sera varied between 93 and 108% for the latex assay compared with 90 and 100% for RIA. Because the procedure involves a titer, the latex agglutination inhibition card test produces results which are categorized rather than results which are continuous. However, it is a rapid and specific method for determining amikacin levels in clinical specimens and is particularly useful when processing small numbers of specimens. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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