| Autor: |
Yoder, B L, Stamm, W E, Koester, C M, Alexander, E R |
| Zdroj: |
Journal of Clinical Microbiology; June 1981, Vol. 13 Issue: 6 p1036-1039, 4p |
| Abstrakt: |
Management of patients potentially infected with Chlamydia trachomatis has been hampered by the cost and time required to perform Chlamydia cultures. To isolate C. trachomatis, we developed a microtiter method that exhibited equal sensitivity but less frequent contamination than our previously used vial-cover slip culture system. In addition, costs and technician time were substantially reduced with the microtest method. Subsequent studies showed that cycloheximide-treated cells were superior to 5-iodo-2-deoxyuridine-treated cells in the microtest method and that a subpassage significantly enhanced the sensitivity of the method. The microtest method appears to be a sensitive, rapid, and economical method for isolating C. trachomatis. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
